 "Step1: Nucleic Acid Extraction </br>(Click to Enlarge)")
Step
1: Nucleic Acid Extraction
With the use of patented Boom silica extraction technology, viral nucleic acids can be isolated in a rapid, convenient manner ensuring their stability for subsequent laboratory manipulations. The method is applicable to a wide variety of sample matrices. The majority of common contaminants experienced in the field, such as albumin, haemoglobin, lipids, DNA, EDTA, heparin, citrate, do not inhibit the accuracy and reproducibility of the assay.
With the use of patented Boom silica extraction technology, viral nucleic acids can be isolated in a rapid, convenient manner ensuring their stability for subsequent laboratory manipulations. The method is applicable to a wide variety of sample matrices. The majority of common contaminants experienced in the field, such as albumin, haemoglobin, lipids, DNA, EDTA, heparin, citrate, do not inhibit the accuracy and reproducibility of the assay.
 "Step2: Nucleic Acid Amplification</br>(Click to Enlarge)")
Step
2: Nucleic Acid Amplification
Convenient and reliable amplification of the target RNA is accomplished under isothermal conditions by the use of three enzymes: The specificity of amplification is ensured by the use of specially designed primers. This combination enables 109-fold amplification of the target RNA sequence in less than two hours. Isothermal incubation (41XC) ensures that no residual DNA contaminants are replicated.
Convenient and reliable amplification of the target RNA is accomplished under isothermal conditions by the use of three enzymes: The specificity of amplification is ensured by the use of specially designed primers. This combination enables 109-fold amplification of the target RNA sequence in less than two hours. Isothermal incubation (41XC) ensures that no residual DNA contaminants are replicated.
Step 3: Signal Detection
 "Step3A Electrochemiluminescent (ECL) detection</br>(Click to Enlarge)")
3A Electrochemiluminescent
(ECL) detection
ECL detection requires the use of a NucliSens ECL Reader. The ECL method enables rapid and accurate detection of the target nucleic acids through the use of two oligonucleotide probes. A capture probe linked to a magnetic bead via a streptavidin-biotin interaction hybridizes with the target RNA. Contaminants are washed away and a second probe labeled with a ruthenium-based tag, binds to another section of the amplified target molecule. The application of an electric current causes the ruthenium tag to emit visible light at 620nm, which is detected spectroscopically. This highly automated ECL process is conducted inside the ECL Reader.
(ECL) detection
ECL detection requires the use of a NucliSens ECL Reader. The ECL method enables rapid and accurate detection of the target nucleic acids through the use of two oligonucleotide probes. A capture probe linked to a magnetic bead via a streptavidin-biotin interaction hybridizes with the target RNA. Contaminants are washed away and a second probe labeled with a ruthenium-based tag, binds to another section of the amplified target molecule. The application of an electric current causes the ruthenium tag to emit visible light at 620nm, which is detected spectroscopically. This highly automated ECL process is conducted inside the ECL Reader.
 "Step3B Microplate (MP) detection<br>(Click to Enlarge)")
3B Microplate (MP) detection
To enhance the specificity of the detection system sequence-specific primers and an additional capture probe are included for confirmation of amplified RNA. In the microplate detection method, an aliquot of the amplification reaction is immobilized by a capture probe attached to a microtitre plate. A detection probe binds to the immobilized amplicon. An enzyme-linked antibody generated the colorimetric signal, which is measured in a standard 96-well microtitre plate spectrophotometer at 405nm.
To enhance the specificity of the detection system sequence-specific primers and an additional capture probe are included for confirmation of amplified RNA. In the microplate detection method, an aliquot of the amplification reaction is immobilized by a capture probe attached to a microtitre plate. A detection probe binds to the immobilized amplicon. An enzyme-linked antibody generated the colorimetric signal, which is measured in a standard 96-well microtitre plate spectrophotometer at 405nm.